Xx Cel Models < 90% OFFICIAL >

| Parameter | Recommended Reporting Standard | Why it matters | |-----------|--------------------------------|----------------| | (donor ID, tissue, passage number) | Cell Line Registry (CLRI), ATCC catalogue number, DOI of source publication | Enables reproducibility and meta‑analysis | | Culture conditions (media, supplements, oxygen, CO₂, temperature) | MIAME‑like checklist for cell culture (MCC) | Subtle changes can alter phenotype | | Authentication (STR profiling, karyotype, mycoplasma test) | ISO 20387:2021, ANSI/ATCC 30‑CFR | Prevents cross‑contamination artifacts | | Phenotypic validation (marker expression, functional assays) | Minimum Information About a Cellular Model (MIACM) | Confirms that the model retains intended biology | | Passage / sub‑culture history | Number of doublings since thaw | Senescence and drift are passage‑dependent | | Genetic modifications (CRISPR guides, plasmid maps, copy number) | Addgene plasmid IDs, CRISPR‑Cas9 validation data | Critical for genotype‑phenotype correlation | | Batch information (lot numbers for media, reagents) | Supplier lot numbers, QC reports | Batch effects are a frequent source of variability | | Data deposition (raw & processed data) | GEO, ArrayExpress, Figshare, Zenodo | Transparency & reuse |

# XX Cel Models – A Practical Guide to Choosing, Using, and Interpreting Modern Cell‑Based Research Systems xx cel models

Choose the “XX Cel model” that most faithfully reproduces the biological question while fitting your budget, timeline, and technical expertise. | Parameter | Recommended Reporting Standard | Why