The key to its function lies in its ability to trap gases. By layering different media or displacing oxygen with an inert gas (like nitrogen or hydrogen), the tube creates a localized oxygen-free environment, allowing anaerobes to grow without requiring expensive vacuum chambers or anaerobic jars.
Today, most labs use or glove boxes for strict anaerobes. However, the Graia’s tube has not entirely disappeared. It is still valued for: graias tube
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