Typically 18–25 bases (min: 15, opt: 20, max: 30). GC Percent: Optimal is 40–60%, which ensures stability. B. Primer Size and Tm Constants
SEQUENCE_ID=TP53_exon7_test SEQUENCE_TEMPLATE=GGACCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTCCCAAGCAATGGATGATTTGATGCTGTCCCCGGACGATATTGAACAATGGTTCACTGAAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGCGTGGCCCCTGCACCAGCAGCTCCTACACCGGCGGCCCCTGCACCAGCCCCCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGCAGCTACGGTTTCCGTCTGGGCTTCTTGCATTCTGGGACAGCCAAGTCTGTGACTTGCACGGTCAGTTGCCCTGAGGGGCTGGCTTCCATGAGACTTCAT primer3 input
Forces the primers to have a G or C at the 3' end to improve binding specificity. Typically 18–25 bases (min: 15, opt: 20, max: 30)
Where you enter your template DNA/RNA in raw format or FASTA format. Typically 18–25 bases (min: 15
This defines the amplicon length.