Pgps3 Unique - Restriction Sites

For seamless assembly (no restriction ligation), cut PGPS3 with a like PstI or NotI . Then:

For molecular biologists, identifying the in pGPS3 is essential for customizing the Transprimer or linearizing the vector for downstream applications. Key Unique Restriction Sites in pGPS3 pgps3 unique restriction sites

A few enzymes that appear in some polylinkers are in PGPS3 because they cut elsewhere in the backbone: For seamless assembly (no restriction ligation), cut PGPS3

If you work with plant transformation, particularly in Arabidopsis thaliana or related species, you’ve likely encountered the vector. Derived from the pGreen series, PGPS3 is a compact, high-copy-number binary vector known for its small size (approximately 3.2 kb), making it ideal for golden gate cloning, site-directed mutagenesis, and efficient transformation into Agrobacterium tumefaciens . For seamless assembly (no restriction ligation)