For most DNA cleanup and miniprep applications, the 1X working solution consists of: (pH 7.5 at 25°C) 80% Ethanol (v/v)
He was on Step 4 of the Plasmid Purification Protocol. The "Wash" step. He had just spun his spin columns, the DNA was sitting pretty on the silica membrane, and the contaminants were washed away. But without the PE buffer—the ethanol-based wash—he couldn't proceed. Without PE, the salt wouldn't leave. Without PE, the DNA wouldn't elute. Without PE, his Monday morning presentation to Dr. Halloway was going to be a very short, very sad confession of failure. qiagen pe buffer recipe
: Mix 1 part 5X base with 4 parts 100% Ethanol to reach the final 80% ethanol concentration. Critical Usage Tips Qiagen Plasmid Prep - Buffer Composition For most DNA cleanup and miniprep applications, the